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Hypercarb provides unique retention and separation of very polar compounds. The surface of Hypercarb is stereo-selective with the capability to separate geometric isomers and …
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Features of Hypercarb columns. Thermo Scientific™ Hypercarb Porous Graphitic Carbon LC Columns offer: Exceptional retention of very polar analytes. Separation for structurally-related substances. pH stablility from 0 to 14, and are not affected by aggressive mobile phases. Ideal solution for high-temperature applications.
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The volatility test consisted of spiking 5 ml of methanol with 50–100 ng of each pesticide and evaporating to dryness under the same conditions of nitrogen flow at ambient temperature. ... 100 ml of non spiked ground water and (d) spiked with 0.5 μg/l of DEA and DIA. Hypercarb column, 10 cm×0.46 cm I.D., acetonitrile gradient with a 5·10 ...
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The Hypercarb cartridge was most promising for extraction of all studied target compounds, but unfortunately, an increase in the sample volume from 10 to 100 mL led to high breakthrough rates. Only triazole sulfonamide was recovered in similar amounts, while less than 20% of 1,2,4-triazole and 1,2,3-triazole were recovered in the eluate (Fig. 2b ).
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Transfer solution to micro-vial for injection.A flow-through fraction from a proteolytic digest wassimulated by diluting a solution containing RGES andDSDPR in Tris buffer (100 mM, pH 8.0) to concentrationsof 0.1 and 0.5 ng/µL respectively.6
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PS-DVB Hypercarb Courtesy V. Coquart and M-C. Henion, J. Chrom., 1992 Retention on Hypercarb columns increases as polarity of the analyte increases, which is the opposite of typical reversed phase materials such as PS-DVB Additional retention is achieved for polar compounds using a Hypercarb column compared to a polar endcapped C18.
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Hypercarb Columns Part of Thermo Fisher Scientific MZ-Analysentechnik GmbH Barcelona-Allee 17 • D-55129 Mainz Tel +49 6131 880 96-0 Fax +49 6131 880 96-20 ... of 0.1 and 0.5 ng/µL respectively. Results and Discussion Following the procedure detailed in the experimental section,
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Hypercarb SPE material is 100% porous graphic carbon (PGC) and offers retention of highly polar compounds that are not usually retained by traditional reversed phase C18 …
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Here, Hypercarb TM excels in separations which are highly troublesome or even impossible using more traditional, silica based columns [12, 13]. The unique separation capabilities of Hypercarb TM are rooted in the diversity of physicochemical interactions between analyte molecules and the graphitic surface. Van der Waals or London forces, …
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(HyperCarb™) The left column is the MRM signal with no added NTP The right hand column is the signal from a spike of 300 ng/ml for AraCTP, 30 ng/ml for CTP and deoxyCTP, 4000 ng/ml for BrATP Crauste et al., (2009) High energy intermediates •Hexose and triose phosphates as well as Krebs cycle intermediates –These are very hydrophilic …
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An ideal method for the analysis of N-glycans would both identify the isomeric structure and deliver a true picture of the relative, if not absolute, amounts of the various structures in one sample. Porous graphitic carbon chromatography coupled with electrospray ionization mass spectrometry (ESI-MS) detection has emerged as a method …
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Thickness (µm) Capacity (ng) 0.10 50-100 0.25 125-250 0.50 250-300 1 500-1000 3 1500-3000 5 2500-5000. Slide 31 Film Thickness and Bleed More stationary phase = More degradation products. Slide 32 Film Thickness and Inertness 1.0 active inactive active inactive 3.0 active inactive 0.25. Slide 33 Film Thickness Summary
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Porous graphitic carbon (PGC, Hypercarb) has unique properties as a stationary phase in high performance liquid chromatography (HPLC).
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The Thermo Hypercarb porous graphite column was the only analytical column that was able to routinely and robustly provide the chromatographic resolution necessary to separate DAC and 2dC. ... The LLOQ was determined to be 2 ng/mL for DAC, 50 ng/mL for 2dC, and 5 ng/mL for 5mC in 3 precision and accuracy runs (Table 1).
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An external calibration method with linear ranges from 10 to 5000 ng/mL for the five target compounds was used for quantification with a correlation coefficients≥0.9973.
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An HPLC column packed with porous graphite Hypercarb™ and a solvent gradient 2-ethyl-1-hexanol (or cyclohexanone) → 1,2,4-trichlorobenzene can separate EVA copolymers over the full range of VA content at temperatures above 100 °C. A linear dependence between the VA content of the copolymers and their elution volume could …
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Hypercarb's porous graphitic surface is not modified. Separation of geometric isomers of Axetil: comparison of a Hypercarb and bonded silica column Comparison of methyl and methylene group selectivity on C18 and Hypercarb columns Hypercarb, 5 µm, 100 x 4.6 mm Part Number: 35005-104630 Mobile Phase: ACN:H 2O:MeOH:Dioxan Isocratic: …
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Porous graphitic carbon (PGC, Hypercarb™) has unique properties as a stationary phase in high-performance liquid chromatography (HPLC). Its chemical surface properties …
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Hypercarb™ HPLC Columns, Thermo Scientific. Porous Graphitic Carbon (PGC) is a unique stationary phase composed of flat sheets of hexagonally arranged carbon atoms with a satisfied valence, as in a very large polynuclear aromatic molecule. Hypercarb is unlike traditional silica bonded phases in both its structure and retentive properties.
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HyperSep Hypercarb SPE Cartridges are packed with 30μm spherical particles of 100% porous graphitic carbon (PGC). Composed of flat …
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Thermo Scientific™ Hypercarb™ グラファイトカーボンカラムをすると、その 100％グラファイトカーボンによりがします。. のがく、な pH やでもしており、がしたのがです ...
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An improved UHPLC-MS/MS assay for the determination of acrylamide from foodstuffs. Author: Kean Woodmansey, Thermo Fisher Scientific, Runcorn, UK Keywords: …
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Hypercarb™ HPLC Columns Technical Guide - Interscience. EN. English Deutsch Français Español Português Italiano Român Nederlands Latina Dansk Svenska Norsk Magyar Bahasa Indonesia Türkçe Suomi Latvian Lithuanian česk ...
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Hypercarb™ column, are the common approaches.6. With both of these approaches, poor method robustness and questionable results are often reported in laboratories, especially when the method is applied in routine high-throughput analysis of samples with rather complex matrix composition. Recent developments in ion chromatography and mass
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The concentrations of U in 63 patients ranged between 4.9 and 36.6 ng/mL. Seventeen patients have concentrations between 14 and 18 ng/mL. Among these, the 2 methods would have proposed different diagnosis for 5 patients (based on the threshold of 16 ng/ml). The difference varied from 1.8 to 5.6 ng/ml.
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carbohydrates were cleaned up using Thermo Scientific Hypercarb SPE. Glycans were eluted from SPE using 40% acetonitrile/60% water/0.1% TFA. Samples were dried in a refrigerated speedvac at ... Triplicate injections of carbohydrate standards (1 ng on-column) using anion exchange chromatography and PAD detection. 0.00 0.50 1.00 1.50 2.00 …
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Catalog number: 35003-031030. Related applications: Chromatography. Technical Support Customer Service. Offer 100% porous graphitic carbon for extended separation …
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A 41.7-μL aliquot of groundwater was then transferred to a polypropylene tube, to which 28.3 μL of HPLC-water, 140 μL of MeOH, and 140 μL of a 6.25 ng mL-1 internal standard solution (prepared in MeOH) were added. The sample was briefly vortexed and a 200-μL aliquot of sample was transferred to a 250-μL polypropylene injection vial.
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hexagonally-arranged carbon atoms (as in a very large polynuclear aromatic molecule). The surface is crystalline and highly. reproducible with a complete absence of micropores. The flat and highly adsorptive surface shows unusual discrimination.
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Retention Effect on Graphite Method (Hypercarb) HyperSep-96 Hypercarb Well Plates Bed Weight Volume Pack Size Part Number 10 mg 1 mL 100 Wells 60302-601 25 mg 1 mL 100 Wells 60302-602 50 mg 1 mL 100 Wells 60302-603 10 mg 1 mL 1 Plate 60302-606 25 mg 1 mL 1 Plate 60302-607 50 mg 1 mL 1 Plate 60302-608 HyperSep SPE Formats …
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